Title: Measures for reducing primary infections in the integrated management of huanglongbing in Sao Paulo citrus belt: technical and economic feasibility
Description: Tematic Project FAPESP process #2017/21460-0. This project aims to evaluate the technical and economic feasibility of available measures to the citrus growers, which could be integrated in order to reduce these primary infections of HLB. Outside the orchard, measures applied directly to control the primary inoculum source will be evaluated. In the orchard edge, up to 200 m from the orchard boundary, the region with the highest concentration of bacteria carrier migrant psyllids from neighboring areas, other measures will be evaluated to bar the primary dispersion into the orchard; prevent the primary inoculation of bacteria by psyllid, preventing the vector access to the plant or its feeding on the plant.
Principal Investigator: Renato Beozzo Bassanezi
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* Activity 1.1. Dynamics of shoots in healthy and HLB-affected trees in commercial orchards, abandoned orchards and backyards * Activity 1.2. Determination of release density and distance of Tamarixia radiata to control Diaphorina citri
| File | Description | Format | Author | Date |
|---|---|---|---|---|
| Climatic conditions | Temperature, RH and rainfall for each experimental period (replications) | .tif | Renato Beozzo Bassanezi | 03/07/2020 |
| Data and formulas (dispersal distance and dispersal area) | Data used to calculate dispersal distance and area | .xlsx | Haroldo Linhares Xavier Volpe | 03/02/2022 |
| Data Tamarixia parasitism, psyllids and parasitoids emerged | Data (all treatments and blocks) used to calculate efficacy of parasitism, psyllids and Tamarixia (f1 generation) emerged (all release density and radii) | .xlsx | Haroldo Linhares Xavier Volpe | 03/02/2022 |
| Parasitism heat map (800, 1600 and 3200 wasps/ha) | Bi-dimensional graphs of the parasitism of Diaphorina citri nymphs by Tamarixia radiata allocated at different radii (7.5, 15, 22.5, and 30 m) from the parasitoid release point. The parasitoid release densities were 800 (a), 1,600 (b), and 3,200 (c) parasitoid wasps/ha. The percentage of parasitized D. citri nymphs was assessed separately for each release radius. “+” indicates the parasitoid release point and black dots represent the places where seedlings with nymphs were attached to ‘Valencia’ sweet orange trees. | .tif | Haroldo Linhares Xavier Volpe | 03/02/2022 |
| Sample unit | Schematic representation of Tamarixia radiata release points (black dots) and points with ‘Valencia’ sweet orange seedlings (red dots) with 35 Diaphorina citri fourth-instar nymphs attached on ‘Valencia’ sweet orange trees located 7.5, 15, 22.5, and 30 m distant (radius – grey circles) from the release point. For each radius, release densities corresponding to 200, 400, 800, 1,600, and 3,200 parasitoid wasps per hectare (densities) were tested in four radii (distances from the parasitoide release pointtotaling 20 treatments. The release points were located equidistantly at 100 m. Light green circles represent ‘Valencia’ sweet orange trees (grove). | .tif | Haroldo Linhares Xavier Volpe | 03/02/2022 |
| File | Description | Format | Author | Date |
|---|---|---|---|---|
| Planting orientation and HLB incidence - Image analysis | Spreadsheet with data of all assessed areas (6 areas) and in two periods. Data corresponded to the total planting holes, removed and present trees, HLB incidence at each distance, cumulative removed trees, and cumulative HLB incidence at each distance for each block and its average. Data of planting direction (parallel or perpendicular to the orcdhard´s edge) are presented separately in each sheet tab. | .xlsx | Renato Beozzo Bassanezi | 16/01/2023 |
| Primiano et al 2022.pdf | proof of the manuscript entitled "The effect of citrus planting orientation on the gradient and incidence of Huanglongbing" - https://doi.org/10.1111/ppa.13704 | Renato Beozzo Bassanezi | 23/07/2024 | |
| row orientation - 1 sticky traps-trial.xlsx | Spreadsheet contains the number of Diaphorina citri adults captured on adhesive sticky traps. Traps were installed in plots with either parallel or perpendicular orientations, located along orchard edges adjacent to sugarcane/soybean fields (Border A) or eucalyptus plantations/natural forest areas (Border B). | .xlsx | Eduardo Augusto Girardi | 03/06/2026 |
| row orientation - 2 Tree assessment - trial.xlsx | This spreadsheet provides counts of trees by category, including healthy (HLB-asymptomatic), diseased (HLB-symptomatic), non-eradicated, eradicated, and other classifications. Data were collected from plots with parallel or perpendicular orientations, situated along orchard edges adjacent to sugarcane/soybean fields (Border A) or eucalyptus plantations/natural forest areas (Border B). | .xlsx | Eduardo Augusto Girardi | 03/06/2026 |
| row orientation - 3 tree growth and canopy measurements | The spreadsheet contains citrus tree growth data collected from June 2022 to August 2024. Variables include treatment orientation (parallel or perpendicular), assessment date, tree age, experimental block, and border position. Tree performance is described by tree height and two canopy-related measurements (diameter and volume). Border treatments A and B are stored in separate sheets within the same spreadsheet. | .xlsx | Eduardo Augusto Girardi | 03/06/2026 |
| tree density - 1 sticky traps-trial.xlsx | Spreadsheet contains the number of Diaphorina citri adults captured on adhesive sticky traps. Traps were installed in plots within edge (0 to 103 m from the edge) and central (103 to 208 m from the edge) areas. The following treatments were allocated in the edge area: grower standard (GS) that corresponded to 855 trees·ha?¹; 50% increase over the GS (GS+50%) that corresponded to 1283 trees·ha?¹; and 100% increase over the GS (GS+100%) that corresponded to 1710 trees·ha?¹. | .xlsx | Eduardo Augusto Girardi | 03/06/2026 |
| tree density - 2 Tree assessment.xlsx | This spreadsheet provides counts of trees by category, including healthy (HLB-asymptomatic), diseased (HLB-symptomatic), non-eradicated, eradicated, and other classifications. Data were collected from plots located at edge (0 to 103 m from the edge) and central (103 to 208 m from the edge) areas. The following treatments were allocated in the edge area: grower standard (GS) that corresponded to 855 trees·ha?¹; 50% increase over the GS (GS+50%) that corresponded to 1283 trees·ha?¹; and 100% increase over the GS (GS+100%) that corresponded to 1710 trees·ha?¹. | .xlsx | Eduardo Augusto Girardi | 03/06/2026 |
| tree density - 3 evaluation of flushes over time.xlsx | The spreadsheet contains citrus flush evaluation data collected on multiple assessment dates from August to February 2024. Variables include experimental block, row, tree, treatment, and canopy quadrant. Flush intensity was visually assessed within each quadrant on each assessment date and recorded using a rating scale from 1 (V1) to 7 (V7). The repeated measurements allow monitoring of flush development over time and comparison of treatment effects across trees, quadrants, and blocks. | .xlsx | Eduardo Augusto Girardi | 03/06/2026 |
| tree density - 4 tree growth and canopy measurements.xlsx | The spreadsheet contains citrus tree growth data collected on multiple assessment dates (from October 2020 to January 2025). Variables include experimental block, treatment, and tree performance measurements. Tree performance is described by tree height and two canopy-related variables (canopy diameter and canopy volume). Measurements are organized by date to allow repeated evaluations over time within each treatment and block combination. | .xlsx | Eduardo Augusto Girardi | 03/06/2026 |
| tree density - 5 fruit yield over time.xlsx | The spreadsheet contains fruit yield data collected over time. Variables include treatment, experimental block, row, planting density (trees per hectare), assessment date, number of trees evaluated, yield per plot, yield per tree, and estimated yield per hectare. The dataset is organized by treatment and block combinations, allowing comparison of yield performance under different planting densities and treatment conditions. | .xlsx | Eduardo Augusto Girardi | 03/06/2026 |
| tree density - 6 fruit quality evaluation.xlsx | The spreadsheet contains citrus fruit quality data collected during January 2024 and November 2025 assessments. Variables include treatment, tree density, experimental block, row, assessment date, juice content (%), fruit weight (g), titratable acidity (g/100 g), juice pH, soluble solids (°Brix), maturity ratio (soluble solids/titratable acidity), and technological index (kg soluble solids per box). The dataset is organized by treatment, tree density, block, and row, allowing comparison of fruit physical and chemical quality attributes under different tree densities. | .xlsx | Eduardo Augusto Girardi | 03/06/2026 |
| File | Description | Format | Author | Date |
|---|---|---|---|---|
| 0 experimental area-trial 1 | Spreadsheet contains the design of the experimental area-trial 1. Yellow cells represent the position of the sticky traps. White cells represent tree holes. | .xlsx | Renato Beozzo Bassanezi | 06/05/2022 |
| 0 experimental area-trial 2.png | Aerial view with the design of the experimental area. Yellow cells represent the position of the sticky traps. In B1, C1, and C2, HLB-affected trees were not removed. In B2, HLB-symptomatic trees were removed. | .png | Renato Beozzo Bassanezi | 05/07/2024 |
| 1 infective psyllid result - lab-trial 2.xlsx | Spreadsheet contains the results of qPCR detection of ‘Candidatus Liberibacter asiaticus’ from psyllids attached in adhesive sticky traps. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 1 infective psyllid results - lab -trial 1 | Spreadsheet contains the results of qPCR detection of ‘Candidatus Liberibacter asiaticus’ from psyllids attached in adhesive sticky traps. | .xlsx | Renato Beozzo Bassanezi | 06/05/2022 |
| 1 psyllid capture on yellow sticky traps - trial 1 | Spreadsheet contains the number of Diaphorina citri adults attached in adhesive sticky traps. | .xlsx | Renato Beozzo Bassanezi | 06/05/2022 |
| 1 psyllid capture on yellow sticky traps-trial 2 | Spreadsheet contains the number of Diaphorina citri adults attached in adhesive sticky traps. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 2 HLB assessment - trail 1 Plot 303 | Spreadsheet contains the number of healthy trees (HLB-asymptomatic tree), diseased tree (HLB-symptomatic tree), non-eradicated tree, eradicated tree, and other categories at the plot 303 of trial 1. | .xlsx | Renato Beozzo Bassanezi | 06/05/2022 |
| 2 HLB assessment - trial 1 Plot 107 | Spreadsheet contains the number of healthy trees (HLB-asymptomatic tree), diseased tree (HLB-symptomatic tree), non-eradicated tree, eradicated tree, and other categories at the plot 107 of trial 1 | .xlsx | Renato Beozzo Bassanezi | 06/05/2022 |
| 2 HLB assessment - trial 1 Plot 108 | Spreadsheet contains the number of healthy trees (HLB-asymptomatic tree), diseased tree (HLB-symptomatic tree), non-eradicated tree, eradicated tree, and other categories at the plot 108 of trial 1. | .xlsx | Renato Beozzo Bassanezi | 06/05/2022 |
| 2 HLB assessment - trial 1 Plot 109 | Spreadsheet contains the number of healthy trees (HLB-asymptomatic tree), diseased tree (HLB-symptomatic tree), non-eradicated tree, eradicated tree, and other categories at the plot 109 in trial 1. | .xlsx | Renato Beozzo Bassanezi | 06/05/2022 |
| 2 HLB assessment - trial 1 Plot 403 | Spreadsheet contains the number of healthy trees (HLB-asymptomatic tree), diseased tree (HLB-symptomatic tree), non-eradicated tree, eradicated tree, and other categories at the plot 403 in trial 1. | .xlsx | Renato Beozzo Bassanezi | 06/05/2022 |
| 3 Production 2015-2018 - trial 1 | Spreadsheet contains the fruit production of each plot in trial 1 from 2015 to 2018.. | .xlsx | Renato Beozzo Bassanezi | 06/05/2022 |
| 3 production-trial 2.xlsx | Spreadsheet contains the fruit production of each plot. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 4 Fruit quality - trial 1 Plot 107 | Spreadsheet contains information of fruit quality from fruit harvested from 2015 to 2017 of the plot 107 of trial 1. | .xls | Renato Beozzo Bassanezi | 06/05/2022 |
| 4 Fruit quality - trial 1 Plot 108 | Spreadsheet contains information of fruit quality from fruit harvested from 2015 to 2019 of the plot 108 of trial 1. | .xls | Renato Beozzo Bassanezi | 06/05/2022 |
| 4 Fruit quality - trial 1 Plot 109 | Spreadsheet contains information of fruit quality from fruit harvested from 2015 to 2019 of the plot 109 of trial 1. | .xls | Renato Beozzo Bassanezi | 06/05/2022 |
| 4 Fruit quality - trial 1 Plot 303 | Spreadsheet contains information of fruit quality from fruit harvested from 2015 to 2019 of the plot 303 of trial 1. | .xls | Renato Beozzo Bassanezi | 06/05/2022 |
| 4 Fruit quality - trial 1 Plot 403 | Spreadsheet contains information of fruit quality from fruit harvested from 2015 to 2019 of the plot 403 of trial 1. | .xls | Renato Beozzo Bassanezi | 06/05/2022 |
| 4 fruit quality -plot 506-trial 2.xlsx | Spreadsheet contains information of fruit quality from fruit harvested in 2020, 2021, 2022, and 2023 of the plot 506. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 4 fruit quality -plot 508A-trial 2.xlsx | 4 fruit quality -plot 508A-trial 2.xlsx | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 4 fruit quality -plot 508-trial 2.xlsx | Spreadsheet contains information of fruit quality from fruit harvested in 2020, 2021, 2022, and 2023 of the plot 508. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| File | Description | Format | Author | Date |
|---|---|---|---|---|
| Primiano et al - manuscript.pdf | Manuscript accepted in Pest Management Science entitled "Ineffectiveness of applying additional insecticide sprays at the border of the citrus block for the control of Huanglongbing" - https://doi.org/10.1002/ps.7281 | Renato Beozzo Bassanezi | 23/07/2024 | |
| Trial 1 - HLB assessment | Spreadsheet contains the new diseased trees (HLB-symptomatic tree) detected in each strip from February 2017 to January 2021 in plots with and without additional insecticide sprays at the edge of citrus plots. Bimonthly assessments are presented separately in each sheet tab. | .xlsx | Renato Beozzo Bassanezi | 06/05/2022 |
| Trial 1 - Plot design | Figure showing the position of yellow stick traps in one plot of experimental area. Green dots: trees without yellow stick trap. Yellow dots: tree that the yellow stick trap was placed. | .tiff | Renato Beozzo Bassanezi | 06/05/2022 |
| Trial 1 - Psyllid assessment | Spreadsheet contains the number of Diaphorina citri adults captured in adhesive sticky traps from September 2015 to January 2021 in plots with and without additional insecticide sprays at the edge of citrus plots. Fortnightly assessments are presented separately in each sheet tab. | .xlsx | Renato Beozzo Bassanezi | 06/05/2022 |
| Trial 1 - Spray coverage | Spreadsheet with spray coverage data in different canopy positions and distances from the citrus plot edge. Experiment was performed with different sprayers and in orchards with different tree canopy size, comparing parallel and perpendicular spraying in relation with planting direction. | .xlsx | Renato Beozzo Bassanezi | 06/05/2022 |
| Trials 2 and 3 - Climatic condition | Temperature and relative air humidity for each experimental period of trial 2 and trial 3 from July to September 2020 carried out in Guaraci – SP, Brazil. | .XLS | Renato Beozzo Bassanezi | 06/05/2022 |
| Trials 2 and 3 - Psyllid mortality | Spread sheet with psyllid mortality data from trial from trial 2 and trial 3 in each sheet tab. Psyllid mortality were assessed in different positions of tree canopy and distances from the citrus plot edge. Border spray applications were made at each side of plot in parallel and perpendicular direction in relation with planting rows. | .xlsx | Renato Beozzo Bassanezi | 06/05/2022 |
| Trials 2 and 3 – Spray coverage | Spread sheet with spray coverage data from trial 2 and trial 3 in each sheet tab. Spray coverage were assessed in different positions of tree canopy and distances from the citrus plot edge. Border spray applications were made at each side of plot in parallel and perpendicular direction in relation with planting rows. | .xlsx | Renato Beozzo Bassanezi | 06/05/2022 |
| File | Description | Format | Author | Date |
|---|---|---|---|---|
| Information - theoretical scenarios.xlsx | Spread sheet with information of the hypothetical orchard at sheet tab “orchard”, data from literature used in our work at sheet tab “data”, an example of how we calculated HLB gradient at sheet tab “HLB gradient calculation-exampl”, and an example of how we calculated total HLB incidence at sheet tab “total HLB incid calculation-ex”. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| Psyllid mortality.xlsx | Spread sheet with psyllid mortality data from trial from trial 1, 2, and 3 in each sheet tab. Psyllid mortality was assessed in different treatments, in which, the treatment corresponded to the confinement day post insecticide spraying. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| File | Description | Format | Author | Date |
|---|---|---|---|---|
| 0 manuscript-Alternate row - Fist Look.pdf | Manuscript published at Plant Disease, entitled "Comparison of insecticide sprays in alternate rows and in all rows on Asian citrus psyllid control and Huanglongbing progress in sweet orange orchards" - https://doi.org/10.1094/PDIS-03-24-0646-RE | Renato Beozzo Bassanezi | 23/07/2024 | |
| Area 2 – Climatic condition | Temperature and relative air humidity for each experimental period of trial 1, trial 2, and trial 3 from September 2020 to February 2021 in Gavião Peixoto – SP, Brazil. | .XLS | Renato Beozzo Bassanezi | 06/05/2022 |
| Area 2 – Psyllid mortality | Spreadsheet with psyllid mortality data from trial 1, trial 2, and trial 3 in each sheet tab. Sprays were done in trees with 2 and 3 years-old, with spray volume of 25 and 40 ml/m3 of the tree canopy, and in alternated rows or in all rows. Spray coverage were assessed in different positions of tree canopy. | .xlsx | Renato Beozzo Bassanezi | 06/05/2022 |
| Area 2 – Spray coverage | Spreadsheet with spray coverage data from trial 1, trial 2, and trial 3 in each sheet tab. Sprays were done in trees with 2 and 3 years-old, with spray volume of 25 and 40 ml/m3 of the tree canopy, and in alternated rows or in all rows. Spray coverage were assessed in different positions of tree canopy. | .xlsx | Renato Beozzo Bassanezi | 06/05/2022 |
| Area 2 – Spray wind speed | Spreadsheet with spray wind speed data of the mounted air blast sprayer from trial 1, trial 2, and trial 3 at different distances from the spray nozzles to different parts of tree canopy. | .xlsx | Renato Beozzo Bassanezi | 06/05/2022 |
| Area 3 – Climatic condition | Temperature and relative air humidity for each experimental period of trial 1, trial 2, and trial 3 from May to September 2021 in Lucianópolis – SP, Brazil. | .XLS | Renato Beozzo Bassanezi | 06/05/2022 |
| Area 3 - Climatic condition 2-sprayers.XLS | Temperature and relative air humidity for each experimental period of psyllid mortality from trial 1, trial 2, and trial 3 that compared the different sprayers from March to June 2022, in Lucianópolis – SP, Brazil. | .XLS | Renato Beozzo Bassanezi | 05/07/2024 |
| Area 3 – Psyllid mortality | Spreadsheet with psyllid mortality data from trial 1, trial 2, and trial 3 in each sheet tab. Sprays were done in trees with 1 year-old, with spray flow rate per spray noozle of 1.1 and 2.2 L/min, and in alternated rows or in all rows. Spray coverage were assessed in different positions of tree canopy. | .xlsx | Renato Beozzo Bassanezi | 06/05/2022 |
| Area 3 - Psyllid mortality 2-sprayers.xlsx | Spreadsheet with psyllid mortality data from trial 1, trial 2, and trial 3 in each sheet tab. Sprays were done in trees with age of 16-17 months, with (T1) multi-row sprayer; (T2) single sided air delivery (air blast sprayer), spraying only one side of the tree; (T3) single sided air delivery (air blast sprayer), spraying both sides of the tree; (T4) double sided air delivery (air blast sprayer), in alternated rows; and (T5) double sided air delivery (air blast sprayer), in all rows. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| Area 3 - Spray coverage | Spreadsheet with spray coverage data from trial 1, trial 2, and trial 3 in each sheet tab. Sprays were done in trees with 1 year-old, with spray flow rate per spray noozle of 1.1 and 2.2 L/min, and in alternated rows or in all rows. Spray coverage were assessed in different positions of tree canopy. | .xlsx | Renato Beozzo Bassanezi | 19/05/2022 |
| Area 3 - Spray coverage 2-sprayers.xlsx | Spreadsheet with spray coverage data from trial 1, trial 2, and trial 3. Sprays were done in trees with age of 16-17 months, with (T1) multi-row sprayer; (T2) single sided air delivery (air blast sprayer), spraying only one side of the tree; (T3) single sided air delivery (air blast sprayer), spraying both sides of the tree; (T4) double sided air delivery (air blast sprayer), in alternated rows; and (T5) double sided air delivery (air blast sprayer), in all rows. Spray coverage were assessed in different positions of tree canopy. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| Area 3 – Spray wind speed | Spreadsheet with spray wind speed data of the mounted air blast sprayer from trial 1, trial 2, and trial 3 at different distances from the spray nozzles to different parts of tree canopy. | .xlsx | Renato Beozzo Bassanezi | 06/05/2022 |
| Area 3 - Spray wind speed 2-sprayers.xlsx | Spreadsheet with spray wind speed data of the mounted air blast sprayer from trial 1, trial 2, and trial 3 at different distances from the spray nozzles to different parts of tree canopy for experiments that compared the different sprayers from March to June 2022, in Lucianópolis – SP, Brazil. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| field trial 1 – HLB assessment.xlsx | Spreadsheet contains the new diseased trees (HLB-symptomatic tree) detected in each strip from September 2017 to February 2022 in citrus block with insecticide sprays performed every 7 days in alternated rows and in citrus block with insecticide sprays performed every 14 days in all rows. Bimonthly assessments are presented separately in each sheet tab. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| field trial 1 - Climatic condition.xlsx | Temperature and relative air humidity for experimental period from Oct, 18th to Nov, 28th 2018 in Mococa/SP, Brazil. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| field trial 1 - Cupper deposition.xlsx | Cupper deposition on leaves assessed in different tree sides (direct and opposite) in relation to spray direction. Sprays were performed in alternated rows and in all rows. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| field trial 1 – Plot design.tif | Aerial view of experimental area. Citrus block T04, T15, T33A, T33B, and T34 received insecticide sprays every 7 days in alternated rows and citrus block T05, T16, T32A, T32B, and T35 received insecticide sprays every 14 days in all rows. | .tif | Renato Beozzo Bassanezi | 05/07/2024 |
| field trial 1 – Psyllid assessment.xlsx | Spreadsheet contains the number of Diaphorina citri adults captured in adhesive sticky traps from February 2017 to February 2022 in citrus block with insecticide sprays performed every 7 days in alternated rows and in citrus block with insecticide sprays performed every 14 days in all rows. Fortnightly assessments are presented separately in each sheet tab. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| field trial 1 – Psyllid mortality.xlsx | Spreadsheet with psyllid mortality data from experiments 1, 2, and 3. Insecticide sprays were performed with dimethoate in alternated rows. Seedlings were placed in different tree sides (direct and opposite) in relation to spray direction. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| field trial 1 – Spray coverage.xlsx | Spreadsheet with spray coverage data. Insecticide sprays were performed in alternated rows or in all rows. Spray coverage were assessed in different positions of tree canopy (top and middle thirds). | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| field trial 2 – HLB assessment.xlsx | Spreadsheet contains the new diseased trees (HLB-symptomatic tree) detected in each strip from April 2021 to April 2023 in citrus block with several spray frequency in alternated row versus the conventional insecticide spray in all rows every 7 days. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| field trial 2 - Climatic condition.xlsx | Temperature and relative air humidity for experimental period from February, 1st to Feb, 2nd 2023 (experiment 1) and March,15th to March, 29th 2023 (experiment 2). | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| field trial 2 – Psyllid assessment.xlsx | Spreadsheet contains the number of Diaphorina citri adults captured in adhesive sticky traps from March 2021 to September 2022 in citrus block with several spray frequency in alternated row versus the conventional insecticide spray in all rows every 7 days. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| field trial 2 – Psyllid mortality.xlsx | Spreadsheet with psyllid mortality data from experiments 1 and 2. Insecticide sprays were performed with the single insecticide formulation of neonicotinoid thiamethoxam and pyrethroid lambda-cyhalothrin. Seedlings were placed in different tree sides (direct and opposite) in relation to spray direction. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| field trial 2 – Spray coverage.xlsx | Spreadsheet with spray coverage data from experiments 1 and 2. Insecticide sprays were performed in alternated rows or in all rows. Water-sensitive papers were placed in different tree sides (direct and opposite) in relation to spray direction. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| File | Description | Format | Author | Date |
|---|---|---|---|---|
| pruning time - 1 citrus flush phenological stage.xlsx | The spreadsheet contains citrus flush evaluation data collected following different pruning time treatments (pre-bloom, petal fall, fruit drop, fruit growth, and control). Variables include pruning time, row, tree, canopy quadrant, and flush assessment scores recorded on multiple assessment dates between September 2020 and March 2024. Flush intensity was evaluated separately within each canopy quadrant and recorded using a visual rating scale. The repeated measurements allow monitoring of vegetative flush development over time and comparison of responses among pruning treatments, trees, and canopy quadrants. A separate worksheet was provided for each commercial orchard block: Q99B T120 (Valencia Americana – Irrigated), Q60B T124 (Valencia – Irrigated), Q115 T145 (Valencia Americana – Rainfed), and Q103 T133 (Valencia – Rainfed). This organization enables the evaluation of pruning effects across different cultivars and water management conditions. | .xlsx | Eduardo Augusto Girardi | 03/06/2026 |
| pruning time - 2 citrus flush growth over time.xlsx | The spreadsheet contains citrus flush shoot development data collected following different pruning time treatments (pre-bloom, petal fall, fruit drop, fruit growth, and control). Variables include treatment, row, tree, canopy side, branch identification number, assessment date, flush developmental stage (EP; V1–V7 scale), flush diameter (cm), and flush length (cm). Measurements were recorded on tagged branches located on different sides of each tree and repeated across multiple assessment dates between September and December 2023. The dataset allows evaluation of flush emergence, developmental progression, and vegetative growth dynamics in response to treatment over time. Flush developmental stage was assessed using the EP scale (V1–V7), while flush diameter and length were measured to quantify shoot growth. Repeated measurements on the same branches enable monitoring of flush growth trajectories and comparison among treatments. A separate worksheet was provided for each commercial orchard block: Q99B T120 (Valencia Americana – Irrigated), Q60B T124 (Valencia – Irrigated), Q115 T145 (Valencia Americana – Rainfed), and Q103 T133 (Valencia – Rainfed). This organization enables evaluation of treatment effects on flush development and shoot growth across different cultivars and water management conditions. | .xlsx | Eduardo Augusto Girardi | 03/06/2026 |
| pruning time - 3 yield, biomass, and tree size.xlsx | The spreadsheet contains citrus tree performance data collected across multiple production seasons. Variables include treatment, experimental block, season, fruit yield (kg/tree), biomass production (g), number of fruit dropped, shoot diameter (cm), tree height (m), and canopy volume (m³/tree). For treated trees, corresponding measurements of control tree height and control canopy volume were also recorded to facilitate comparisons with untreated trees. The treatments were applied at different phenological stages, including pre-bloom, petal fall, fruit drop, and fruit growth, along with an untreated control. Data were collected across multiple experimental blocks and production seasons, allowing evaluation of treatment effects on yield, vegetative growth, canopy development, biomass accumulation, and fruit retention over time. A separate worksheet was provided for each commercial orchard block: Q99B T120 (Valencia Americana – Irrigated), Q60B T124 (Valencia – Irrigated), Q115 T145 (Valencia Americana – Rainfed), and Q103 T133 (Valencia – Rainfed). This organization enables comparisons of treatment responses across cultivars and irrigation regimes, providing insight into the effects of management practices under contrasting production environments. | .xlsx | Eduardo Augusto Girardi | 03/06/2026 |
| pruning time - 4 fruit quality assessments.xlsx | The spreadsheet contains citrus fruit quality data collected across multiple phenological treatments, experimental blocks, and production seasons. Variables include treatment, block, season, juice content (%), fruit weight (g), titratable acidity (%), juice pH, soluble solids (°Brix), maturity ratio (SS/TA), and technological index (kg SS per box). The dataset is organized by phenological treatment timing, including pre-bloom, petal fall, fruit drop, fruit growth, and an untreated control. Data were collected across multiple experimental blocks and seasons (2020–21 to 2023–24), allowing evaluation of treatment effects on fruit physical and chemical quality parameters under different orchard conditions. A separate worksheet was provided for each commercial orchard block: Q99B T120 (Valencia Americana – Irrigated), Q60B T124 (Valencia – Irrigated), Q115 T145 (Valencia Americana – Rainfed), and Q103 T133 (Valencia – Rainfed). This structure enables comparison of fruit quality responses across cultivars and irrigation regimes, supporting evaluation of management effects under contrasting production environments. | .xlsx | Eduardo Augusto Girardi | 03/06/2026 |
| Top pruning - Data.xlsx | The spreadsheet contains data from a citrus top-pruning experiment comparing pruned and non-pruned trees. Assessments are organized into separate worksheets and include measurements related to tree growth, vegetative flushing, psyllid populations, HLB incidence, and fruit yield. | .xlsx | Eduardo Augusto Girardi | 03/06/2026 |
| Tree vigor - Data.xlsx | The spreadsheet contains data from a citrus tree vigor experiment comparing trees grafted on vigorous and dwarfing rootstocks. Assessments are organized into separate worksheets and include measurements related to tree growth, vegetative flushing, psyllid populations, psyllid dispersal following release events, HLB incidence, and fruit yield. | .xlsx | Eduardo Augusto Girardi | 03/06/2026 |
| File | Description | Format | Author | Date |
|---|---|---|---|---|
| 1.2 Tamarixia Custo de produção.xlsx | Spreadsheet contains the steps to produce Tamarixia radiata. Each tab is a step. "I" means initial investment and "A" means annual cost. The last tab is the summary and result | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 1.2 Tamarixia LIberação.xlsx | Spreadsheet contains information on the costs required for release Tamarixia radiata, operational performance of each releasing method and economic analysis | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 2.1 Plantio isca experimento.xlsx | Spreadsheet contains information on the costs required for the experiment's activities, the outcome of the experiment, eradication costs, agrochemical prices, operating costs in each year of analysis, production from the third year onwards, NPV analysis, summary and interest rate used in the NPV | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 2.1 Plantio isca incidência 2023.xlsx | Spreadsheet contains information on the costs required for the experiment's activities, the outcome of the experiment considering HLB incidence of 2023 in SP citrus belt, eradication costs, agrochemical prices, operating costs in each year of analysis, production from the third year onwards, NPV analysis, summary and interest rate used in the NPV | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 2.1 Plantio isca ponto de virada.xlsx | Spreadsheet contains information on the costs required for the experiment's activities, the outcome of the experiment considering considering HLB incidence at the turning point, eradication costs, agrochemical prices, operating costs in each year of analysis, production from the third year onwards, NPV analysis, summary and interest rate used in the NPV | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 2.1 Plantio isca sem sistêmico.xlsx | Spreadsheet contains information on the costs required for the experiment's activities without considering the application of systemic insecticide, the outcome of the experiment, eradication costs, agrochemical prices, operating costs in each year of analysis, production from the third year onwards, NPV analysis, summary and interest rate used in the NPV | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 2.2 Adensamento - análise econômica.xlsx | Spreadsheet contains information on the costs required for the experiment's activities, the prices of agrochemicals, the results of the experiment, operating costs in each year of analysis, production from the third year onwards and NPV analysis. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 2.2 Adensamento - custo com erradicação.xlsx | Spreadsheet contains information on the costs required to eradicate a plant, operating yields, the cost of inspection, cutting and replanting and a final summary. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 2.2 Direção de plantio.xlsx | Spreadsheet contains information on the costs required to eradicate a plant, operational yields, experiment data, the cost of inspection, cutting, replanting and a final summary. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 2.3 Erradicação Guaraci.xlsx | Spreadsheet contains information on costs needed to eradicate a plant, operating yields, experiment data, cost of inspection, cutting and replanting, NPV and interest rate used in NPV. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 2.3 Erradicação Reginópolis.xlsx | Spreadsheet contains information on costs needed to eradicate a plant, operating yields, experiment data, cost of inspection, cutting and replanting, NPV and interest rate used in NPV. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 2.3 erradicação Ubirajara.xlsx | Spreadsheet contains information on costs needed to eradicate a plant, operating yields, experiment data, cost of inspection, cutting and replanting, NPV and interest rate used in NPV. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 3.1 entorno.xlsx | Spreadsheet contains information on the costs required for spraying, the prices of agrochemicals, the data from the experiment and the cost of spraying. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 3.3 Ruas alternadas análise econômica.xlsx | Spreadsheet contains information on the costs required for spraying, the prices of agrochemicals, the cost of spraying and the NPV analysis, cost benefit and interest rate used in the NPV. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 3.3 Ruas alternadas erradicação.xlsx | Spreadsheet contains information on the costs required for the experiment's activities, agrochemical prices, experiment data, operating costs, expected revenue and economic analysis. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 3.4 Época de poda.xlsx | Spreadsheet contains information on the costs required for the pruning operation, the data and results of the experiment, the expected costs and income in each year of the study, the NPV and the summary. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 3.4 Porta-enxerto erradicação.xlsx | Spreadsheet contains information on the costs required to eradicate a plant, operating yields, the cost of inspection, cutting and replanting and a final summary. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 3.4 Porta-enxerto análise econômica.xlsx | Spreadsheet contains information on the costs required for the experiment's activities, agrochemical prices, experiment data, operating costs, expected revenue and economic analysis. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 3.6 Caulim Borborema experimento.xlsx | Spreadsheet contains information on the costs required for the experiment's activities, experiment data, eradication costs, agrochemical prices, operating costs and expected revenue in each year of analysis, NPV analysis and summary. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 3.6 Caulim Borborema simulações.xlsx | Spreadsheet contains information on the costs required for the experiment activities, the experiment data with the simulation modifications, eradication costs, agrochemical prices, operating costs and expected revenue in each year of analysis, NPV analysis and summary. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 3.6 Caulim Casa Branca experimento.xlsx | Spreadsheet contains information on the costs required for the experiment's activities, experiment data, eradication costs, agrochemical prices, operating costs and expected revenue in each year of analysis, NPV analysis and summary. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
| 3.6 Caulim Casa Branca simulações.xlsx | Spreadsheet contains information on the costs required for the experiment activities, the experiment data with the simulation modifications, eradication costs, agrochemical prices, operating costs and expected revenue in each year of analysis, NPV analysis and summary. | .xlsx | Renato Beozzo Bassanezi | 05/07/2024 |
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